It’s been a hot minute since last update on this lab note page. Things since last write up had been quite hectic, both in good and bad sense.

While on the usual internet lam traveling down the endless piles of papers reporting on fascinating new mysteries from the microbial world, an MRA stood out and caught my attention.

Last year’s experience working with MacLea lab brought with it quite the varied ensemble of microbial sequencing side-projects, involving both long and short read platforms.

It’s finally that time of the year – our annual Halobacteria mutant strain genome sequencing and assembly. Quick recap; our lab has an acidophilic/tolerant (robust growth at pH 4.0~5.0) Halobacteria mutant strain from around 2019.

It’s six in the evening where I am – sitting aside a window looking out into a field of almost complete darkness.

As people say, time flies. Unbearable heat of the summer at the warehouse lab, compounded by emanations from overheating computers are still vivid in memory as if it was yesterday. Now, I’m looking at the last of autumn leaves as the flora sheds their colors and turn gaunt one by one.

I had an idea to look into the history of academic posters while working on the Nanopore Community Meeting 2022 material – almost a whole year ago now.

It’s a special week over at Binomica Labs.

Alphabet representation for protein amino acid residues can fun to play around with – let’s see what a season’s greeting looks like with a quick blast search: Dumping above into NCBI blast shows: Interesting! I’m surprised we got any hits at all – let’s look at the alignment quality: Ah – this is no good.

2022 finally draws to a close with coming of an unusually warm and humid first week of December in the city.

Here’s a quick test running Dorado basecaller on ONT long read files in a Google Colab GPU instance, based on previous notes written by Miles Benton.