The cultures of red-tinted bacteria (hopefully a bacteriochlorophyll utilizing species of some sort) from previous East River sampling run were getting a little long in the tooth, so I subcultured them into the new, smaller autoclavable plates with better seal using the last of the PPES-K media.
The initial test recorded in the blue shift DNA mutagenesis test II entry failed, most likely due to the state of the competent E.coli. We ran the same experiment described in the previous post again, with freshly prepared NEB turbo competent E.coli.
The blue shifted plasmid samples from yesterday’s test was taken out of the red light exposure chamber and transformed with NEB turbo E.coli strain. Hopefully the full 24 hour exposure was enough to induce purine mutation in the naked DNA, we’ll find out soon.
We’re running a quick and dirty test of methylene blue based DNA mutagensis method (based off their photosensitive quality) today, something to occupy our mind while waiting for the new system 76 rig to arrive (for our upcoming mutant Halobacterium NRC-1 genome sequencing project). The test is a simple one involving methylene blue diluent and […]
I decided to do a quick pigmentation extraction and spectral sweep test for the East River bacterial sample B and D today, with older Halobacteria NRC-1 cultures acting as controls. The extraction protocol is the same one I described in a previous post.
Another delayed post, with a bit of a hectic schedule.
The day is finally here!
A rather busy week, I’m trying to finish up the final draft of our Deinococcus research paper before the year is done, so we can get with formatting and other final touches necessary to publish it officially early next year.
Quick update on the day’s activity before the weekend. Growth comparison of the halomedia fungi contamination against YPD media yielded a somewhat disappointing result. The fungi seems to thrive in YPD with far slower growth rate in regular and acidic halomedia we have in stock.
The main media used in our lab for Halobacteria NRC-1 propagation is ATCC 213, consisting of below components for a 500ml batch NaCl 125g MgSO4 5g KCl 2.5g CaCl2 0.1g Yeast Extract 5g Tryptone 1.25g The media straight after mixing usually has a pH of 5.9~ or so. I keep two batches at a time, […]
I ran out of the original iteration of the kitchen media built around PPES-II using store bought milk and soy protein powder. The original media performed above expectations for the East River environmental sampling, but not quite so with the regular propagation of our in-house model organisms, notably brewer’s yeast and Deinococcus radiophilus.
